Blooms of sequence-specific culturable bacteria in the sea

Ann-Sofi Rehnstam; Stina Bäckman; David C. Smith; Farooq Azam; Åke Hagström

doi:10.1111/j.1574-6968.1993.tb05806.x

Blooms of sequence-specific culturable bacteria in the sea

Ann-Sofi Rehnstam, Stina Bäckman, David C. Smith, Farooq Azam, Åke Hagström

Research output: Contribution to journal › Article › peer-review

123 Citations (Scopus)

Abstract

Using specific deoxyoligonucleotide probes we have discovered seasonally strong (up to ∼ 100%) dominance of bacteria hybridizing to a single probe, in near shore waters off Scripps pier (32°53′N; 117°15′W). The probes were designed from partially sequenced 16S rRNA (V3 domain) of isolated marine bacteria. The results indicate that this approach may be used for studies of bacterial populations in the marine environment. We have shown that a number of genotypes that at times are dominant in the natural assemblages are culturable (and not, ‘viable-but-unculturable’). Additionally, our data suggests that the discrepancy between viable counts and direct counts in sea water samples can be explained by low plating efficiency.

Original language	English
Pages (from-to)	161-166
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	102
Issue number	3-4
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb05806.x
Publication status	Published - 1993
Externally published	Yes

Swedish Standard Keywords

Microbiology (10606)

Keywords

Bloom
Colony forming
DNA probe
Identification
Marine bacterium

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/j.1574-6968.1993.tb05806.x

123 Citations
1 Doctoral Thesis

Use of 16S ribosomal RNA probes for the detection of marine bacteria
Rehnstam-Holm, A-S., 1994, Univ..
Research output: Types of Thesis › Doctoral Thesis

Cite this

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title = "Blooms of sequence-specific culturable bacteria in the sea",

abstract = "Using specific deoxyoligonucleotide probes we have discovered seasonally strong (up to ∼ 100%) dominance of bacteria hybridizing to a single probe, in near shore waters off Scripps pier (32°53′N; 117°15′W). The probes were designed from partially sequenced 16S rRNA (V3 domain) of isolated marine bacteria. The results indicate that this approach may be used for studies of bacterial populations in the marine environment. We have shown that a number of genotypes that at times are dominant in the natural assemblages are culturable (and not, {\textquoteleft}viable-but-unculturable{\textquoteright}). Additionally, our data suggests that the discrepancy between viable counts and direct counts in sea water samples can be explained by low plating efficiency.",

keywords = "Bloom, Colony forming, DNA probe, Identification, Marine bacterium",

author = "Ann-Sofi Rehnstam and Stina B{\"a}ckman and Smith, {David C.} and Farooq Azam and {\AA}ke Hagstr{\"o}m",

year = "1993",

doi = "10.1111/j.1574-6968.1993.tb05806.x",

language = "English",

volume = "102",

pages = "161--166",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "3-4",

}

TY - JOUR

T1 - Blooms of sequence-specific culturable bacteria in the sea

AU - Rehnstam, Ann-Sofi

AU - Bäckman, Stina

AU - Smith, David C.

AU - Azam, Farooq

AU - Hagström, Åke

PY - 1993

Y1 - 1993

N2 - Using specific deoxyoligonucleotide probes we have discovered seasonally strong (up to ∼ 100%) dominance of bacteria hybridizing to a single probe, in near shore waters off Scripps pier (32°53′N; 117°15′W). The probes were designed from partially sequenced 16S rRNA (V3 domain) of isolated marine bacteria. The results indicate that this approach may be used for studies of bacterial populations in the marine environment. We have shown that a number of genotypes that at times are dominant in the natural assemblages are culturable (and not, ‘viable-but-unculturable’). Additionally, our data suggests that the discrepancy between viable counts and direct counts in sea water samples can be explained by low plating efficiency.

AB - Using specific deoxyoligonucleotide probes we have discovered seasonally strong (up to ∼ 100%) dominance of bacteria hybridizing to a single probe, in near shore waters off Scripps pier (32°53′N; 117°15′W). The probes were designed from partially sequenced 16S rRNA (V3 domain) of isolated marine bacteria. The results indicate that this approach may be used for studies of bacterial populations in the marine environment. We have shown that a number of genotypes that at times are dominant in the natural assemblages are culturable (and not, ‘viable-but-unculturable’). Additionally, our data suggests that the discrepancy between viable counts and direct counts in sea water samples can be explained by low plating efficiency.

KW - Bloom

KW - Colony forming

KW - DNA probe

KW - Identification

KW - Marine bacterium

U2 - 10.1111/j.1574-6968.1993.tb05806.x

DO - 10.1111/j.1574-6968.1993.tb05806.x

M3 - Article

VL - 102

SP - 161

EP - 166

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3-4

ER -

Blooms of sequence-specific culturable bacteria in the sea

Abstract

Swedish Standard Keywords

Keywords

UN SDGs

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Research output

Use of 16S ribosomal RNA probes for the detection of marine bacteria

Cite this