Cytokines in gingival crevicular fluid in elderly rheumatoid arthritis patients in a population-based cross-sectional study: RANTES was associated with periodontitis

Maria K Söderlin, Gösta Rutger Persson, Stefan Renvert, Johan Sanmartin Berglund

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Abstract

OBJECTIVE: We studied cytokines in gingival crevicular fluid (GCF) in a cross-sectional population-based cohort of rheumatoid arthritis (RA) patients ≥61 years of age with and without a diagnosis of periodontitis.

BACKGROUND DATA: Earlier studies on cytokines in GCF in RA patients have not given clear results.

METHODS: In a population-based cross-sectional study of patients ≥61 years of age, 233 RA patients were identified. 132 (57%) dentate RA patients participated. All participants received rheumatological and dental examinations, and had a panoramic radiograph taken. GCF was sampled on each patient. Interleukins 1-β (IL-1β), IL-4, IL-6, IL-10, IL-17A, tumor necrosis factor alpha (TNF-α), interferon gamma (IFN-γ), and chemokines RANTES/CCL5, eotaxin and monocyte chemoattractant protein (MCP-1) were analyzed in GCF. These cytokines were stratified for periodontitis, age, gender, body mass index (BMI), smoking, and anti-cyclic citrullinated protein (anti-CCP) status. Binary logistic regression analyses with periodontitis as outcome were performed adjusting for the above mentioned confounding factors including anti-rheumatic medication, disease duration and the cytokine in question.

RESULTS: Periodontitis was diagnosed in 80/132 (61%) of study participants. The 110 RA patients not participating were older, had a higher mean erythrocyte sedimentation rate (ESR), had a higher mean DAS28ESR (Disease Activity Score 28 using ESR) and were less often on biologic treatment. Only RANTES was associated with periodontitis (p = .049, OR 1.001, 95% CI 1.000-1.002) in the binary logistic regression analyses.

CONCLUSION: In this population-based elderly RA cohort, neither pro-inflammatory nor anti-inflammatory cytokines in GCF were clearly associated with a diagnosis of periodontitis.

Original languageEnglish
Pages (from-to)907-916
Number of pages10
JournalJournal of Periodontal Research
Volume56
Issue number5
Early online date2021
DOIs
Publication statusPublished - 2021

Swedish Standard Keywords

  • Rheumatology and Autoimmunity (30210)

Keywords

  • cytokines
  • gingival crevicular fluid
  • periodontitis
  • rheumatoid arthritis
  • Cross-Sectional Studies
  • Periodontitis
  • Gingival Crevicular Fluid/chemistry
  • Humans
  • Arthritis, Rheumatoid/drug therapy
  • Chemokine CCL5/analysis
  • Aged
  • Cytokines/analysis

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