High throughput screening method to explore protein interactions with nanoparticles

Irem Nasir, Warda Fatih, Anja Svensson, Dennis Radu, Sara Linse, Celia Cabaleiro-Lago, Martin Lundqvist

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the screening method may reveal changes in colloidal stability of nanomaterials depending on the physicochemical conditions.

Original languageEnglish
JournalPLoS ONE
Volume10
Issue number8
DOIs
Publication statusPublished - 2015
Externally publishedYes

Swedish Standard Keywords

  • Biochemistry and Molecular Biology (10602)

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