The effect of nanoparticles on amyloid aggregation depends on the protein stability and intrinsic aggregation rate.

Celia Cabaleiro-Lago, Olga Szczepankiewicz, Sara Linse

Research output: Contribution to journalArticlepeer-review

117 Citations (Scopus)

Abstract

Nanoparticles interfere with protein amyloid formation. Catalysis of the process may occur due to increased local protein concentration and nucleation on the nanoparticle surface, whereas tight binding or a large particle/protein surface area may lead to inhibition of protein aggregation. Here we show a clear correlation between the intrinsic protein stability and the nanoparticle effect on the aggregation rate. The results were reached for a series of five mutants of single-chain monellin differing in intrinsic stability toward denaturation, for which a correlation between protein stability and aggregation propensity has been previously documented by Szczepankiewicz et al. [Mol. Biosyst.20107 (2), 521-532]. The aggregation process was monitored by thioflavin T fluorescence in the absence and presence of copolymeric nanoparticles with different hydrophobic characters. For mutants with a high intrinsic stability and low intrinsic aggregation rate, we find that amyloid fibril formation is accelerated by nanoparticles. For mutants with a low intrinsic stability and high intrinsic aggregation rate, we find the opposite--a retardation of amyloid fibril formation by nanoparticles. Moreover, both catalytic and inhibitory effects are most pronounced with the least hydrophobic nanoparticles, which have a larger surface accessibility of hydrogen-bonding groups in the polymer backbone.

Original languageEnglish
Pages (from-to)1852-1857
Number of pages5
JournalLangmuir
Volume28
Issue number3
DOIs
Publication statusPublished - 2012
Externally publishedYes

Swedish Standard Keywords

  • Physical Chemistry (10402)

Keywords

  • Alpha-B-Crystallin
  • Beta-peptide
  • Fibril formation
  • Solid-liquid interfaces
  • adsorption
  • conformational-changes
  • inhibition
  • invitro
  • molecular chaperone
  • nanoparticles
  • polymeric

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