Jämförelse av kemiinstrument och validering av referensintervall hos hund och katt

Translated title of the thesis: Comparison of chemical instruments and validation of reference intervals in dogs and cats
  • Johanna Borg

Student thesis: Bachelor

Abstract

Clinical chemical analyzes has high clinical relevance. In serum/plasma, different parameters can be quantified. Parameters can be proteins, enzymes, ions, metals, lipids, and carbohydrates. With reference intervals, veterinarians can set diagnosis, follow treatment and development of the disease. Parameters are detected with different analysis principles/methods; colorimetry, immunoturbidimetry, enzymatic method and potentiometry. The animal hospital, AniCura, in Hässleholm accept dogs and cats. About 120 chemical analyzes are analyzed every day. AniCura purchased a new instrument, Indiko Plus, which will replace Cobas C111. Indiko Plus provide, more sample positions, 8 new analyzes, increased capacity, and facilitated use.

The purpose of this study was to compare the instruments and produce a new reference interval which was compared to the reference interval provided by Thermo Fisher. To verify Indiko Plus, a precision and linearity study were conducted. Blood sampling of dogs and cats was performed by staff at AniCura. The comparison was made with patient samples and the reference intervals were obtained using samples from healthy animals.

The comparison showed 9 of 13 analyzes had a statistically significant difference. The reason for this is probably due to the difference in reagents, the age of the instruments and the time between measurements. A new reference interval was developed and did not differ much from the Thermo Fisher interval. Further validation due to low population was recommended. The precision for Indiko Plus was approved. The linearity study shows not linear trend but was likely due to a bad pipette and should be redone.

Date of Award2021-Jun-22
Original languageSwedish
SupervisorSandra Fagerlund (Supervisor), Celia Cabaleiro Lago (Supervisor) & Ann-Sofi Rehnstam-Holm (Examiner)

Educational program

  • Bachelor of Science in Biomedical Laboratory Science

Courses and Subjects

  • Biomedical Laboratory Science

University credits

  • 15 HE credits

Swedish Standard Keywords

  • Biochemistry and Molecular Biology (10602)
  • Clinical Laboratory Medicine (30223)

Keywords

  • indiko plus
  • cobas c111
  • clinical chemistry
  • dog
  • cat

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