Mätning av kluven Kaspas-3 och kluven PARP i manganbehandlade prostatacancerceller

Translated title of the thesis: Mesurement of cleaved Caspase-3 and cleaved PARP in manganese-treated prostate cancer cells
  • Hussein Karim Ali

Student thesis: Bachelor

Abstract

Prostate cancer is the sixth most common cancer type in the world, and the third most common cancer type among men. The different types of treatments that are available today do not usually cure the disease. It is therefore important to develop better treatment methods. It has previously been stated that manganese can cause apoptosis in different cell types. It provides an opportunity to use manganese to inhibit cancer and it is therefore important to know which apoptotic markers that are involved. The purpose of the project was to investigate whether an increase in the apoptotic markers cleaved Kaspas-3 and cleaved PARP after manganese treatment of prostate cancer cells. Thereafter, it can determine whether manganese treatment has caused cell death by inducing apoptosis. During the project prostate cancer cells (PC3-cells) were cultivated, then treated with 200 µM manganese for 6, 24 and 48 hours. The amount of the proteins cleaved Kaspas-3 and cleaved PARP was measured by using a sandwich ELISA kit. A clear gradual increase of apoptotic markers with incubation time was expected. The expected result was not obtained, for cleaved Kaspase-3 manganese treatment even decreased the concentrations. There may have been problems with the performance of the analysis such as poor lysis of the cells or uneven growth of the cells. More studies are required to investigate this and other potential apoptotic markers.

Date of Award2019-Feb-22
Original languageSwedish
SupervisorBodil Hernroth (Supervisor), Helena Tassidis (Supervisor) & Ann-Sofi Rehnstam-Holm (Examiner)

Educational program

  • Bachelor of Science in Biomedical Laboratory Science

University credits

  • 15 HE credits

Swedish Standard Keywords

  • Biomedical Laboratory Science/Technology (30402)

Keywords

  • prostate cancer
  • cancer treatment
  • manganese
  • cleaved caspase-3
  • cleaved parp
  • sandwich-elisa

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