Uttryck av apoptotiska proteiner vid manganbehandling av prostatacancer cellinjer

  • Alice Nilsson

    Student thesis: Bachelor

    Abstract

    Malaria is a parasitic disease known worldwide. In 2015 the disease caused 438000 deaths of which 90% occurred in the African region.

    The parasites destroy the erythrocytes in the human body and are transmitted by the Anopheles mosquitoes. There are five species of the genus Plasmodium that causes malaria. The species P. falciparum causes the most deaths and therefore most diagnostics are about detecting this species.

    Malaria occurs approximately a week after the infecting bite. The symptoms are often varying and nonspecific and hence complicate the diagnosis of the disease.

    Thick and thin blood film microscopy examination (Pheripheral blood smears) is the golden standard for malaria diagnosis. However, to be able to make quicker and more reliable diagnoses new methods need to be developed. Even though there are effective drugs against malaria, delayed diagnoses and treatment are major causes of death in many countries. Knowledge and resources are primarily lacking in the endemic areas where it is needed the most. There are many criteria for a method that should be fulfilled; such as sensitivity, rapidness, accuracy and cost-effectiveness. The method needs to be easy to use in the actual environment and it has to have potential for the future. This is a comparative study of microscopy of thick and thin blood smears (PBS), quantitative buffy coat (QBC), polymerase chain reaction (PCR), cell-microarray chip, loop-mediated isothermal amplification (LAMP) and rapid diagnostic tests (RDTs).

    The conclusion that can be drawn from this study is that more work needs to be done before a new method can replace conventional microscopy. In the endemic areas the aspects of cost and simplicity are important to take into consideration. Among the methods in this study, The LAMP seems to be the one with the most potential if it continues to develop in the future.

    Date of Award2016-Aug-09
    Original languageSwedish
    SupervisorHelena Tassidis (Supervisor) & Bodil Hernroth (Examiner)

    Educational program

    • Bachelor of Science in Biomedical Laboratory Science

    University credits

    • 15 HE credits

    Swedish Standard Keywords

    • Biomedical Laboratory Science/Technology (30402)

    Keywords

    • malaria
    • diagnosis
    • plasmodium
    • method

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