Emergence of blaNDM-5 Enterobacterales in Swedish Wastewater Effluent

Carolina Axelsson; Annie Justh De Neczpal; Åsa Sjöling; Ann Sofi Rehnstam-Holm

doi:10.1017/S0950268826101071

Emergence of blaNDM-5 Enterobacterales in Swedish Wastewater Effluent

Carolina Axelsson
, Annie Justh De Neczpal
, Åsa Sjöling
, Ann Sofi Rehnstam-Holm^*

^*Huvudförfattare för detta arbete

Fakulteten för naturvetenskap

Forskningsoutput: Tidskriftsbidrag › Artikel › Peer review

Sammanfattning

Carbapenem-resistant Enterobacterales were isolated from the outlet of a wastewater treatment plant in Kristianstad in southern Sweden, during spring and summer of the year 2024. MALDI-ToF MS identification and subsequent whole-genome sequencing identified eight Klebsiella pneumoniae strains belonging to ST437 and ST873 and 10 Escherichia coli strains belonging to ST167, ST648, ST1284, and ST8346. All strains, except K. pneumoniae ST873, were NDM-5 positive. K. pneumaniae ST437 and E. coli ST8346 carried two carbapenemase genes, bla NDM-5 and bla OXA-181, as well as the extended-spectrum-β-lactamases (ESBL) gene bla CTX-M-15. These two multi-drug-resistant ST variants that are widespread globally, that have previously not been detected in clinical settings in Sweden, are now detected in treated wastewater in a Swedish middle-sized town.

Originalspråk	Engelska
Tidskrift	Epidemiology and Infection
Volym	154
DOI	https://doi.org/10.1017/S0950268826101071
Status	Publicerad - 2026-jan.-30

Åtkomst av dokument

10.1017/S0950268826101071

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@article{36b0aac255d9449e826acceb9033810a,

title = "Emergence of blaNDM-5 Enterobacterales in Swedish Wastewater Effluent",

abstract = "Carbapenem-resistant Enterobacterales were isolated from the outlet of a wastewater treatment plant in Kristianstad in southern Sweden, during spring and summer of the year 2024. MALDI-ToF MS identification and subsequent whole-genome sequencing identified eight Klebsiella pneumoniae strains belonging to ST437 and ST873 and 10 Escherichia coli strains belonging to ST167, ST648, ST1284, and ST8346. All strains, except K. pneumoniae ST873, were NDM-5 positive. K. pneumaniae ST437 and E. coli ST8346 carried two carbapenemase genes, bla NDM-5 and bla OXA-181, as well as the extended-spectrum-β-lactamases (ESBL) gene bla CTX-M-15. These two multi-drug-resistant ST variants that are widespread globally, that have previously not been detected in clinical settings in Sweden, are now detected in treated wastewater in a Swedish middle-sized town. ",

keywords = "Bacterial Proteins/genetics, Drug Resistance, Multiple, Bacterial/genetics, Escherichia coli/genetics, Klebsiella pneumoniae/genetics, Microbial Sensitivity Tests, Sweden, Wastewater/microbiology, Whole Genome Sequencing, beta-Lactamases/genetics",

author = "Carolina Axelsson and \{Justh De Neczpal\}, Annie and {\AA}sa Sj{\"o}ling and Rehnstam-Holm, \{Ann Sofi\}",

year = "2026",

month = jan,

day = "30",

doi = "10.1017/S0950268826101071",

language = "English",

volume = "154",

journal = "Epidemiology and Infection",

issn = "0950-2688",

publisher = "Cambridge University Press",

}

TY - JOUR

T1 - Emergence of blaNDM-5 Enterobacterales in Swedish Wastewater Effluent

AU - Axelsson, Carolina

AU - Justh De Neczpal, Annie

AU - Sjöling, Åsa

AU - Rehnstam-Holm, Ann Sofi

PY - 2026/1/30

Y1 - 2026/1/30

N2 - Carbapenem-resistant Enterobacterales were isolated from the outlet of a wastewater treatment plant in Kristianstad in southern Sweden, during spring and summer of the year 2024. MALDI-ToF MS identification and subsequent whole-genome sequencing identified eight Klebsiella pneumoniae strains belonging to ST437 and ST873 and 10 Escherichia coli strains belonging to ST167, ST648, ST1284, and ST8346. All strains, except K. pneumoniae ST873, were NDM-5 positive. K. pneumaniae ST437 and E. coli ST8346 carried two carbapenemase genes, bla NDM-5 and bla OXA-181, as well as the extended-spectrum-β-lactamases (ESBL) gene bla CTX-M-15. These two multi-drug-resistant ST variants that are widespread globally, that have previously not been detected in clinical settings in Sweden, are now detected in treated wastewater in a Swedish middle-sized town.

AB - Carbapenem-resistant Enterobacterales were isolated from the outlet of a wastewater treatment plant in Kristianstad in southern Sweden, during spring and summer of the year 2024. MALDI-ToF MS identification and subsequent whole-genome sequencing identified eight Klebsiella pneumoniae strains belonging to ST437 and ST873 and 10 Escherichia coli strains belonging to ST167, ST648, ST1284, and ST8346. All strains, except K. pneumoniae ST873, were NDM-5 positive. K. pneumaniae ST437 and E. coli ST8346 carried two carbapenemase genes, bla NDM-5 and bla OXA-181, as well as the extended-spectrum-β-lactamases (ESBL) gene bla CTX-M-15. These two multi-drug-resistant ST variants that are widespread globally, that have previously not been detected in clinical settings in Sweden, are now detected in treated wastewater in a Swedish middle-sized town.

KW - Bacterial Proteins/genetics

KW - Drug Resistance, Multiple, Bacterial/genetics

KW - Escherichia coli/genetics

KW - Klebsiella pneumoniae/genetics

KW - Microbial Sensitivity Tests

KW - Sweden

KW - Wastewater/microbiology

KW - Whole Genome Sequencing

KW - beta-Lactamases/genetics

U2 - 10.1017/S0950268826101071

DO - 10.1017/S0950268826101071

M3 - Article

C2 - 41614306

AN - SCOPUS:105029647675

SN - 0950-2688

VL - 154

JO - Epidemiology and Infection

JF - Epidemiology and Infection

ER -

Emergence of blaNDM-5 Enterobacterales in Swedish Wastewater Effluent

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