Functional Characterization of the Interaction between the Superantigen Staphylococcal Enterotoxin A and the TCR

Per Antonsson, Anette Gjörloff Wingren, Johan Hansson, Terje Kalland, Mikael Varga, Mikael Dohlsten

Forskningsoutput: TidskriftsbidragArtikelPeer review

30 Citeringar (Scopus)

Sammanfattning

In this report, we show that despite an overall amino acid residue identity of more than 80% between the staphylococcal enterotoxins (SE) A and E, these proteins markedly differ in their absolute requirement for the MHC class II during T cell activation. The superantigens were produced as C215Fab-SE fusion proteins and analyzed for their ability to activate T cells in a MHC class II-independent manner, using C215 Ag expressing cell lines as pseudo super-APCs. C215Fab-SEA, but not C215Fab-SEE, induced T cell cytotoxicity and proliferation in these MHC class II-independent systems. Introduction of a region from SEA, comprising amino acids 20-27, to SEE transferred the ability to engage T cells in the absence of MHC class II. Analysis of the Vβ specificity of the chimeric SEA/SEE molecules and a panel of SEA mutants demonstrated that the site for TCR interaction covers the edge surrounding the shallow cavity on top of the SEA molecule.

OriginalspråkEngelska
Sidor (från-till)4245-4251
Antal sidor7
TidskriftJournal of Immunology
Volym158
Nummer9
StatusPublicerad - 1997-maj-01
Externt publiceradJa

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  • Biologi (106)

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