High throughput screening method to explore protein interactions with nanoparticles

Irem Nasir, Warda Fatih, Anja Svensson, Dennis Radu, Sara Linse, Celia Cabaleiro-Lago, Martin Lundqvist

Forskningsoutput: TidskriftsbidragArtikelPeer review

9 Citeringar (Scopus)

Sammanfattning

The interactions of biological macromolecules with nanoparticles underlie a wide variety of current and future applications in the fields of biotechnology, medicine and bioremediation. The same interactions are also responsible for mediating potential biohazards of nanomaterials. Some applications require that proteins adsorb to the nanomaterial and that the protein resists or undergoes structural rearrangements. This article presents a screening method for detecting nanoparticle-protein partners and conformational changes on time scales ranging from milliseconds to days. Mobile fluorophores are used as reporters to study the interaction between proteins and nanoparticles in a high-throughput manner in multi-well format. Furthermore, the screening method may reveal changes in colloidal stability of nanomaterials depending on the physicochemical conditions.

OriginalspråkEngelska
TidskriftPLoS ONE
Volym10
Utgåva8
DOI
StatusPublicerad - 2015
Externt publiceradJa

Nationell ämneskategori

  • Biokemi och molekylärbiologi (10602)

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