Forskningsoutput per år
Forskningsoutput per år
Ann-Sofi Rehnstam, Anders Norqvist, Hans Wolf-Watz, Åke Hagström
Forskningsoutput: Tidskriftsbidrag › Artikel › Peer review
16S rRNA from seven different Vibrio anguillarum strains was partially sequenced and compared. From this sequence information we could design a 25-base-long oligonucleotide and use it as a specific probe for identification of V. anguillarum. This was determined by RNA-DNA colony hybridization and slot-blot hybridization. Strong, specific hybridization to the probe was observed for all V. anguillarum strains tested. Furthermore, no cross-hybridization could be seen against five other bacterial species. The detection limit was 5 x 10(3) bacteria per ml. It was even possible to detect V. anguillarum, by slot-blot hybridization, directly in a homogenized kidney from a fish that had died of vibriosis. The partial sequence information revealed small but significant differences between strains of the same species. These sequence differences are sufficiently significant to allow serotyping on the RNA level. Comparing strains of different serotypes revealed a 10-base and an 11-base difference in V. anguillarum serotypes O8 and O9, respectively, in a 122-base partial sequence.
Originalspråk | Engelska |
---|---|
Sidor (från-till) | 1907-1910 |
Antal sidor | 3 |
Tidskrift | Applied and Environmental Microbiology |
Volym | 55 |
Nummer | 8 |
Status | Publicerad - 1989 |
Externt publicerad | Ja |
Forskningsoutput: Typer av avhandlingar › Doktorsavhandling