Interfacing antibody-based microarrays and digital holography enables label-free detection for loss of cell volume

Background: We introduce the combination of digital holographic microscopy (DHM) and antibody microarrays as a powerful tool to measure morphological changes in specifically antibody-captured cells. The aim of the study was to develop DHM for analysis of cell death of etoposide-treated suspension cells. Results/methodology: We demonstrate that the cell number, mean area, thickness and volume were noninvasively measured by using DHM. The cell number was stable over time, but the two cell lines showed changes of cell area and cell irregularity after treatment. The cell volume in etoposide-treated cells was decreased, whereas untreated cells showed stable volume. Conclusion: Our results provide proof of concept for using DHM combined with antibody-based microarray technology for detecting morphological changes in captured cells. We use an innovative technique that combines digital holographic microscopy and the capture of cells with antibody microarrays as a powerful tool to measure cellular morphological changes. With this technique, the cells can be noninvasively viewed in 3D over time. We obtain results showing changes in cellular parameters including cell area, thickness and volume of the captured cells after treatment with the cell death-inducing drug etoposide. The cell volume in etoposide-treated cells showed a decrease, while untreated cells remained stable. Digital holographic microscopy combined with antibody microarray technology can be a future method for detecting morphological changes in treated cancer cells.