Steroid hormone determination in water using an environmentally friendly membrane based extraction technique

Saioa Zorita, Pär Hallgren, Lennart Mathiasson

    Forskningsoutput: TidskriftsbidragArtikelPeer review

    56 Citeringar (Scopus)

    Sammanfattning

    In this study, a method was developed for determination of steroid hormones (17beta-estradiol, estrone, 17alpha-ethynylestradiol) in tap and sewage water samples from Sweden. Sample preparation and analysis were performed by a hollow-fibre microporous membrane liquid-liquid extraction (HF-MMLLE) set-up combined with gas chromatography-mass spectrometry (GC-MS). In this approach, only the organic liquid in the lumen (10microL) of the hollow-fibre membrane was utilised for depleting extraction. Several parameters were studied, including: type of organic solvent, sample pH, salt and humic acid content. The optimised method allowed the determination of the analyte at the low ngL(-1) level in tap and sewage water. A linear plot gave correlation coefficients better than 0.995 and resulted in a method limit of detection of 1.6, 3 and 10ngL(-1) for 17beta-estradiol, estrone, and 17alpha-ethynylestradiol, respectively, in sewage water. Enrichment factors were over 1400 after derivatisation. The repeatabilities at 50 and 600ngL(-1) were better than 10% and 6%, respectively.

    OriginalspråkEngelska
    Sidor (från-till)1-8
    Antal sidor7
    TidskriftJournal of Chromatography A
    Volym1192
    Utgåva1
    DOI
    StatusPublicerad - 2008

    Nationell ämneskategori

    • Biokemi och molekylärbiologi (10602)
    • Naturvetenskap (1)

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